Human NELF ELISA Kit
Cat no : KE00949
Synonyms
NSMF, Nasal embryonic LHRH factor, Nasal embryonic luteinizing hormone-releasing hormone factor, NMDA receptor synaptonuclear signaling and neuronal migration factor
Validation Data Gallery
Product Information
KE00949 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human NELF ELISA kit is to be used to detect and quantify protein levels of endogenous human NELF. The assay recognizes human NELF. An antibody specific for human NELF has been pre-coated onto the microwells. The human NELF protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human NELF is added to detect the captured human NELF protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human NELF ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 2.0 pg/mL |
| Range | 62.5-4000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 26012 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 103% | 95%-111% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 2,072.8 | 22.7 | 1.1 |
| 2 | 8 | 486.6 | 13.7 | 2.8 |
| 3 | 8 | 258.8 | 10.1 | 3.9 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 2,066.6 | 37.6 | 1.8 |
| 2 | 16 | 475.1 | 19.0 | 4.0 |
| 3 | 16 | 252.2 | 11.5 | 4.6 |
Background Information
NSMF, also named as NELF (Nasal embryonic LHRH factor), is a 530 amino acid transcription factor involved in the migration of LHRH neurons. NELF is an attractive candidate gene for a role in GnRH neuron migration, mammalian puberty, and the pathophysiology of Kallmann syndrome (KS). NELF as a nuclear protein involved in the developmental function of the reproductive axis.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | NSMF, Nasal embryonic LHRH factor, Nasal embryonic luteinizing hormone-releasing hormone factor, NMDA receptor synaptonuclear signaling and neuronal migration factor |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.