Human NGAL ELISA Kit
Cat no : KE00144
Synonyms
24p3, HNL, LCN2, lipocalin 2, NGAL, Oncogene 24p3, p25
Validation Data Gallery
Product Information
KE00144 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The NGAL ELISA kit is to be used to detect and quantify protein levels of endogenous NGAL. The assay recognizes human NGAL. An antibody specific for NGAL has been pre-coated onto the microwells. The NGAL protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for NGAL is added to detect the captured NGAL protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm .
| Product name | Human NGAL ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell cuture supernatants, Urine, Saliva |
| Assay type | Sandwich |
| Sensitivity | 3.3 pg/mL |
| Range | 78.1-5000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3934 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 93% | 79%-106% |
| cell cuture supernatants | 88% | 73%-103% |
| urine | 92% | 84%-102% |
| saliva | 97% | 75%-113% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 2,493.7 | 166.0 | 6.7 |
| 2 | 20 | 655.1 | 16.6 | 2.5 |
| 3 | 20 | 154.8 | 11.0 | 7.1 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 2,550.7 | 78.4 | 3.1 |
| 2 | 24 | 625.4 | 17.5 | 2.8 |
| 3 | 24 | 150.1 | 7.4 | 4.9 |
Background Information
Neutrophil gelatinase-associated lipocalin (NGAL) also known as Lipocalin-2 (LCN2), is a secreted glycoprotein that belongs to the lipocalin protein family. NGAL exists as a monomer, a homodimer and a disulfide-linked heterodimer in cells, covalently conjugated with gelatinase (matrix metalloproteinase-9, MMP-9) in order to decrease its degradation. Several roles of NGAL was identified, including iron trafficking, chemotactic, and promote cell differentiation and proliferation. NGAL was originally found in human neutrophils, and expressed in tissues containing renal, uterus, liver, spleen, as well as cardiomyocytes, neurons and immune cells. Studies showed that NGAL is a significant biomarker of cardiovascular diseases, ischemic stroke, acute renal injury, inflammation and metabolic diseases, and it can be detected in human serum, saliva and urine.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | 24p3, HNL, LCN2, lipocalin 2, NGAL, Oncogene 24p3, p25 |
Publications
| Species | Sample Type | Title |
|---|---|---|
J Crit Care A translational study of Galectin-3 as an early biomarker and potential therapeutic target for ischemic-reperfusion induced acute kidney injury. | ||