Human NME2 ELISA Kit
Cat no : KE00948
Synonyms
C-myc purine-binding transcription factor PUF, EC:2.7.13.3, EC:2.7.4.6, Histidine protein kinase NDKB, NDKB
Validation Data Gallery
Product Information
KE00948 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human NME2 ELISA kit is to be used to detect and quantify protein levels of endogenous human NME2. The assay recognizes human NME2. An antibody specific for human NME2 has been pre-coated onto the microwells. The human NME2 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human NME2 is added to detect the captured human NME2 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human NME2 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.22 ng/mL |
| Range | 1.95-125 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 4831 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 76% | 73%-83% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 59.96 | 3.30 | 5.50 |
| 2 | 8 | 16.63 | 1.37 | 8.21 |
| 3 | 8 | 4.61 | 0.37 | 8.10 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 60.23 | 3.20 | 5.31 |
| 2 | 16 | 16.49 | 1.37 | 8.28 |
| 3 | 16 | 4.44 | 0.39 | 8.76 |
Background Information
NME2(non-metastatic cells 2, protein), also named NDKB(Nucleoside diphosphate kinase B) and NM23B, belongs to the NDK family. It is involved in the phosphorylation of nucleoside diphosphates, supplying nucleotide pools for DNA/RNA synthesis, and interacts with membrane receptors and constitutes a novel molecular regulatory mechanism of GPCR endocytosis. It negatively regulates Rho activity by interacting with AKAP13/LBC, and also acts as a transcriptional activator of the MYC gene and binds DNA non-specifically.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | C-myc purine-binding transcription factor PUF, EC:2.7.13.3, EC:2.7.4.6, Histidine protein kinase NDKB, NDKB |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.