Human NRG1, isoform Alpha ELISA Kit
Cat no : KE00572
Synonyms
NRG1, NRG1A, ARIA, GGF, GGF2
Validation Data Gallery
Product Information
KE00572 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human NRG1, isoform Alpha ELISA kit is to be used to detect and quantify protein levels of endogenous human NRG1, isoform Alpha. The assay recognizes human NRG1, isoform Alpha. An antibody specific for human NRG1, isoform Alpha has been pre-coated onto the microwells. The human NRG1, isoform Alpha protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human NRG1, isoform Alpha is added to detect the captured human NRG1, isoform Alpha protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human NRG1, isoform Alpha ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.05 ng/mL |
| Range | 0.156-10 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3084 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 81% | 79%-84% |
| Cell lysate | 110% | 98%-121% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 4.15 | 0.18 | 4.31 |
| 2 | 8 | 1.97 | 0.05 | 2.30 |
| 3 | 8 | 1.95 | 0.03 | 1.73 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 5.18 | 0.37 | 7.13 |
| 2 | 16 | 1.23 | 0.06 | 4.64 |
| 3 | 16 | 0.64 | 0.03 | 4.62 |
Background Information
NRG1 (Neuregulin-1) belongs to the neuregulin family of growth factors responsible for cardiac development and cardiac protection from stress and is a protein that plays a central role in cell signaling in the heart, mammary gland, and central nervous system. NRG1 has been implicated in neural development, neurotransmission, and synaptic plasticity. NRG1 and its receptor ErbB tyrosine kinase are expressed not only in the developing nervous system, but also in the adult brain. NRG1 has multiple isoforms that are generated by usage of different promoters and alternative splicing of a single gene.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | NRG1, NRG1A, ARIA, GGF, GGF2 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
Publications
| Species | Sample Type | Title |
|---|---|---|
Neural Regen Res Macrophages-derived NRG-1 promotes angiogenesis after ischemic stroke via the Akt-mTOR pathway. |