Human NSMCE2 ELISA Kit
Cat no : KE00958
Synonyms
C8orf36, E3 SUMO protein ligase NSE2, E3 SUMO-protein ligase NSE2, E3 SUMO-protein transferase NSE2, EC:2.3.2.-
Validation Data Gallery
Product Information
KE00958 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human NSMCE2 ELISA kit is to be used to detect and quantify protein levels of endogenous human NSMCE2. The assay recognizes human NSMCE2. An antibody specific for human NSMCE2 has been pre-coated onto the microwells. The human NSMCE2 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human NSMCE2 is added to detect the captured human NSMCE2 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human NSMCE2 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.03 ng/mL |
| Range | 0.156-10 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 286053 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 97% | 95%-101% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 5.06 | 0.22 | 4.35 |
| 2 | 8 | 1.54 | 0.06 | 3.84 |
| 3 | 8 | 0.48 | 0.02 | 5.24 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 4.94 | 0.26 | 5.34 |
| 2 | 16 | 1.49 | 0.07 | 4.86 |
| 3 | 16 | 0.48 | 0.02 | 4.28 |
Background Information
NSMCE2 (E3 SUMO-protein ligase NSE2) has a C-terminal Siz/PIAS-RING (SP-RING) domain with E3 SUMO ligase activity. NSMCE2 is a SUMO ligase required for DNA repair and prevention of DNA damage-induced apoptosis. NSMCE2, an essential SUMO ligase of the SMC5/6 complex suppresses cancer and aging in mice.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | C8orf36, E3 SUMO protein ligase NSE2, E3 SUMO-protein ligase NSE2, E3 SUMO-protein transferase NSE2, EC:2.3.2.- |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.