Human NSUN2 ELISA Kit
Cat no : KE00640
Synonyms
EC:2.1.1.-, EC:2.1.1.-, EC:2.1.1.203, mRNA cytosine C(5)-methyltransferase, Myc-induced SUN domain-containing protein, RNA cytosine C(5)-methyltransferase NSUN2
Validation Data Gallery
Product Information
KE00640 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human NSUN2 ELISA kit is to be used to detect and quantify protein levels of endogenous human NSUN2. The assay recognizes human NSUN2. An antibody specific for human NSUN2 has been pre-coated onto the microwells. The human NSUN2 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human NSUN2 is added to detect the captured human NSUN2 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human NSUN2 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 19.4 pg/mL |
| Range | 78.1-5000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 54888 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 81% | 70%-90% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 2521.6 | 83.6 | 3.3 |
| 2 | 8 | 635.6 | 27.8 | 4.4 |
| 3 | 8 | 303.3 | 11.1 | 3.7 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 2556.2 | 91.2 | 3.6 |
| 2 | 16 | 623.8 | 27.1 | 4.3 |
| 3 | 16 | 316.7 | 17.0 | 5.4 |
Background Information
NSUN2, also known as SAKI or Misu (Myc-induced SUN-domain-containing protein), is an RNA methyltransferase belonging to the NSUN (NOL1/NOP2/Sun) family of proteins, which are characterized by their conserved S-adenosylmethionine (SAM)-dependent methyltransferase domain. It catalyzes the methylation of cytosine residues (m⁵C) in transfer RNAs (tRNAs) and other RNA molecules, playing a crucial role in post-transcriptional RNA modification.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | EC:2.1.1.-, EC:2.1.1.-, EC:2.1.1.203, mRNA cytosine C(5)-methyltransferase, Myc-induced SUN domain-containing protein, RNA cytosine C(5)-methyltransferase NSUN2 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.