Human Nectin 3 ELISA Kit
Cat no : KE00996
Synonyms
Nectin-3, PVRL3, CD113, Nectin cell adhesion molecule 3, poliovirus receptor related 3
Validation Data Gallery
Product Information
KE00996 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human Nectin 3 ELISA kit is to be used to detect and quantify protein levels of endogenous human Nectin 3. The assay recognizes human Nectin 3. An antibody specific for human Nectin 3 has been pre-coated onto the microwells. The human Nectin 3 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human Nectin 3 is added to detect the captured human Nectin 3 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human Nectin 3 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.24 ng/mL |
| Range | 0.78-50 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 25945 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 96% | 90%-106% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 24.13 | 2.32 | 9.63 |
| 2 | 8 | 5.96 | 0.46 | 7.67 |
| 3 | 8 | 3.01 | 0.29 | 9.77 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 22.56 | 3.08 | 13.64 |
| 2 | 16 | 6.16 | 0.56 | 9.12 |
| 3 | 16 | 3.23 | 0.36 | 11.16 |
Background Information
Nectin 3, also known as CD113, is a member of the nectin family of cell adhesion molecules. It plays a crucial role in cell-cell adhesion and the formation of adherens junctions, which are essential for maintaining tissue integrity and cell polarity. Nectin 3 can form homophilic and heterophilic interactions, meaning it can bind to itself or other nectin family members, such as Nectin-2. These interactions are important for processes like cell migration, tissue development, and immune cell transmigration.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Nectin-3, PVRL3, CD113, Nectin cell adhesion molecule 3, poliovirus receptor related 3 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.