Human Nectin-4 ELISA Kit
Cat no : KE00346
Synonyms
Ig superfamily receptor LNIR, LNIR, nectin 4, poliovirus receptor related 4, PRR4, PVRL4
Validation Data Gallery
Product Information
KE00346 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Nectin-4 ELISA kit is to be used to detect and quantify protein levels of endogenous Nectin-4. The assay recognizes human Nectin-4. An antibody specific for Nectin-4 has been pre-coated onto the microwells. The Nectin-4 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-Nectin-4 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human Nectin-4 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | serum, plasma, cell culture supernatant, urine, cell lysate |
| Assay type | Sandwich |
| Sensitivity | 4.1 pg/mL |
| Range | 15.6-1000 pg/ml |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 81607 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 100% | 88%-113% |
| Urine | 92% | 83%-105% |
| Cell culture supernatant | 102% | 91%-120% |
| Cell lysate | 88% | 76%-100% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 523.1 | 11.5 | 2.2 |
| 2 | 20 | 119.8 | 5.4 | 4.5 |
| 3 | 20 | 29.5 | 2.5 | 8.1 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 508.0 | 11.9 | 2.3 |
| 2 | 24 | 117.8 | 2.7 | 2.3 |
| 3 | 24 | 29.6 | 2.2 | 7.4 |
Background Information
Nectin-4, also known as PVRL4, is a type-I transmembrane glycoprotein that belongs to the nectin subfamily of immunoglobulin-like adhesion molecules that participate in Ca(2+)-independent cell-cell adhesion. The extracellular domain of Nectin-4, which contains three immunoglobulin-like domains (V and two C2-type domains, VCC), can be proteolytically cleaved to release a soluble form. Nectin-4 interacts with afadin via its carboxyl-terminal cytoplasmic sequence and trans-interacts with nectin-1/PRR1 through V domain interaction. It acts as a receptor for measles virus. Nectin-4 is overexpressed in multiple human malignancies and the aberrant expression is correlated with cancer progression and poor prognostic.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Ig superfamily receptor LNIR, LNIR, nectin 4, poliovirus receptor related 4, PRR4, PVRL4 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.