Speedy™ Human Nectin-4 One-Step ELISA Kit
Cat no : SE50058
Synonyms
PVRL4, Ig superfamily receptor LNIR, LNIR, nectin 4, Nectin cell adhesion molecule 4
Validation Data Gallery
Product Information
| Product name | Speedy™ Human Nectin-4 One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant, Urine, Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.8 pg/mL |
| Range | 15.6-1000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 81607 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 111% | 103%-117% |
| Cell culture supernatant | 108% | 98%-119% |
| Urine | 101% | 97%-106% |
| Cell lysate | 107% | 102%-127% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 515.5 | 30.8 | 6.0 |
| 2 | 8 | 122.3 | 3.8 | 3.1 |
| 3 | 8 | 36.7 | 1.1 | 3.0 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 496.1 | 29.5 | 5.9 |
| 2 | 16 | 119.6 | 4.1 | 3.4 |
| 3 | 16 | 35.6 | 1.5 | 4.2 |
Background Information
Nectin-4, also known as PVRL4, is a type-I transmembrane glycoprotein that belongs to the nectin subfamily of immunoglobulin-like adhesion molecules that participate in Ca(2+)-independent cell-cell adhesion. The extracellular domain of Nectin-4, which contains three immunoglobulin-like domains (V and two C2-type domains, VCC), can be proteolytically cleaved to release a soluble form. Nectin-4 interacts with afadin via its carboxyl-terminal cytoplasmic sequence and trans-interacts with nectin-1/PRR1 through V domain interaction. It acts as a receptor for measles virus. Nectin-4 is overexpressed in multiple human malignancies and the aberrant expression is correlated with cancer progression and poor prognostic.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | PVRL4, Ig superfamily receptor LNIR, LNIR, nectin 4, Nectin cell adhesion molecule 4 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
2、If thermostatic shaker is not available, will it affect the duration of my experiment and the experimental data?
Answer: If thermostatic shaker is not available, it may take 2.5 hours to complete this experiment. After adding the antibody cocktail, we suggest you incubate the plate at 37℃ for 2 hours. After adding TMB, you only need to incubate the plate at 37℃ for 15 minutes. Through comparative experiments, our R&D personnel have confirmed that you can still obtain valid experimental data, although the signal will be slightly reduced.