Human OAT ELISA Kit
Cat no : KE00937
Synonyms
EC:2.6.1.13, GACR, HOGA, OATASE, OKT
Validation Data Gallery
Product Information
KE00937 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human OAT ELISA kit is to be used to detect and quantify protein levels of endogenous human OAT. The assay recognizes human OAT. An antibody specific for human OAT has been pre-coated onto the microwells. The human OAT protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human OAT is added to detect the captured human OAT protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human OAT ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.58 ng/mL |
| Range | 3.13-200 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 4942 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 74% | 70%-77% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 101.37 | 3.80 | 3.75 |
| 2 | 8 | 26.62 | 0.66 | 2.50 |
| 3 | 8 | 13.23 | 0.75 | 5.67 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 99.87 | 3.99 | 4.00 |
| 2 | 16 | 26.15 | 1.04 | 3.99 |
| 3 | 16 | 13.15 | 0.56 | 4.23 |
Background Information
Ornithine-delta-aminotransferase (OAT) is a pyridoxal-5' phosphate dependent mitochondrial matrix enzyme. It controls the L-ornithine (Orn) level in tissues by catalysing the transfer of the delta-amino group of Orn to 2-oxoglutarate. OAT is widely present in all organisms and participates in stress-induced Pro accumulation in the cytoplasm.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | EC:2.6.1.13, GACR, HOGA, OATASE, OKT |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.