Human OCT1 ELISA Kit
Cat no : KE00993
Synonyms
NF A1, NF-A1, Oct-1, Octamer-binding protein 1, Octamer-binding transcription factor 1
Validation Data Gallery
Product Information
KE00993 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human OCT1 ELISA kit is to be used to detect and quantify protein levels of endogenous human OCT1. The assay recognizes human OCT1. An antibody specific for human OCT1 has been pre-coated onto the microwells. The human OCT1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human OCT1 is added to detect the captured human OCT1 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human OCT1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.12 ng/mL |
| Range | 0.78-50 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 5451 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 88% | 84%-96% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 27.59 | 0.83 | 3.01 |
| 2 | 8 | 6.48 | 0.26 | 3.95 |
| 3 | 8 | 3.35 | 0.18 | 5.48 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 26.31 | 1.60 | 6.06 |
| 2 | 16 | 6.57 | 0.34 | 5.20 |
| 3 | 16 | 3.53 | 0.27 | 7.75 |
Background Information
OCT1 (also known as POU2F1) is a ubiquitously expressed transcription factor belonging to the POU-domain family, playing a crucial role in various biological processes. It is involved in the regulation of gene expression, cell differentiation, and proliferation. For instance, it is essential for T cell differentiation, and its overexpression is associated with poor prognoses in certain cancers. Additionally, it can cooperate with other transcription factors to promote specific cellular functions. In the context of skeletal muscle gene expression, OCT1 is identified as an upstream regulator involved in transcriptional regulation and cellular homeostasis.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | NF A1, NF-A1, Oct-1, Octamer-binding protein 1, Octamer-binding transcription factor 1 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.