Human OLIG2 ELISA Kit
Cat no : KE00797
Synonyms
5O7, bHLHb1, bHLHe19, Class B basic helix-loop-helix protein 1, Class E basic helix-loop-helix protein 19
Validation Data Gallery
Product Information
KE00797 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human OLIG2 ELISA kit is to be used to detect and quantify protein levels of endogenous human OLIG2. The assay recognizes human OLIG2. An antibody specific for human OLIG2 has been pre-coated onto the microwells. The human OLIG2 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human OLIG2 is added to detect the captured human OLIG2 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human OLIG2 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.05 ng/mL |
| Range | 0.156-10 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 10215 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 107% | 79%-118% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 4.59 | 0.16 | 3.39 |
| 2 | 8 | 1.15 | 0.07 | 6.26 |
| 3 | 8 | 0.59 | 0.02 | 3.62 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 4.67 | 0.17 | 3.65 |
| 2 | 16 | 1.14 | 0.07 | 6.36 |
| 3 | 16 | 0.60 | 0.02 | 4.05 |
Background Information
Olig1 and Olig2 are sonic hedgehog (Shh)-responsible basic helix-loop-helix (bHLH) transcription factors, and are initially identified as oligodendrocyte lineage-specific transcription factors. Olig2 transcription factor is widely expressed throughout the central nervous system. OLIG2 is involved in the development and progression of some brain tumors, particularly gliomas.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | 5O7, bHLHb1, bHLHe19, Class B basic helix-loop-helix protein 1, Class E basic helix-loop-helix protein 19 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.