Human OPTN/Optineurin ELISA Kit
Cat no : KE00445
Synonyms
Optineurin,OPTN,ALS12,E3-14.7K-interacting protein
Validation Data Gallery
Product Information
KE00445 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human OPTN/Optineurin ELISA kit is to be used to detect and quantify protein levels of endogenous human OPTN/Optineurin. The assay recognizes human OPTN/Optineurin. An antibody specific for human OPTN/Optineurin has been pre-coated onto the microwells. The human OPTN/Optineurin protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human OPTN/Optineurin is added to detect the captured human OPTN/Optineurin protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human OPTN/Optineurin ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 17.8 pg/mL |
| Range | 93.8-6000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 10133 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 77% | 72%-81% |
| Cell lysate | 72% | 70%-73% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 2,995.0 | 155.5 | 5.2 |
| 2 | 8 | 798.9 | 30.2 | 3.8 |
| 3 | 8 | 419.4 | 17.5 | 4.2 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 2,968.6 | 161.0 | 5.4 |
| 2 | 16 | 775.2 | 51.8 | 6.7 |
| 3 | 16 | 398.4 | 30.8 | 7.7 |
Background Information
OPTN (Optineurin) is a crucial autophagy receptor that facilitates the recognition of infected pathogens, protein aggregates, and mitochondria. The depletion of OPTN significantly increases the formation of protein aggregates and results in a loss of function of selective autophagy. In contrast, upregulating the expression of OPTN can induce autophagy. OPTN is associated with many human disorders that are closely related to autophagy, such as rheumatoid arthritis, osteoporosis, and nephropathy.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Optineurin,OPTN,ALS12,E3-14.7K-interacting protein |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.