Human Osteopontin/OPN ELISA Kit
Cat no : KE00375
Synonyms
SPP1,BNSP,Bone sialoprotein 1,BSPI,ETA 1
Validation Data Gallery
Product Information
KE00375 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human Osteopontin/OPN ELISA kit is to be used to detect and quantify protein levels of endogenous human Osteopontin/OPN. The assay recognizes human Osteopontin/OPN. An antibody specific for human Osteopontin/OPN has been pre-coated onto the microwells. The human Osteopontin/OPN protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human Osteopontin/OPN is added to detect the captured human Osteopontin/OPN protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human Osteopontin/OPN ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant, Urine, Human milk, Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.07 ng/mL |
| Range | 0.156-10 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 6696 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Urine | 109% | 87%-123% |
| Human milk | 112% | 103%-121% |
| Cell lysate | 96% | 72%-114% |
| Cell culture supernatant | 83% | 75%-98% |
| Human plasma | 85% | 79%-89% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 10.58 | 0.93 | 8.76 |
| 2 | 20 | 2.46 | 0.10 | 4.20 |
| 3 | 20 | 0.57 | 0.02 | 3.25 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 9.99 | 0.97 | 9.66 |
| 2 | 24 | 2.26 | 0.13 | 5.65 |
| 3 | 24 | 0.54 | 0.04 | 7.14 |
Background Information
Osteopontin (OPN), also known as SPP1, is a secreted glycophosphoprotein that belongs to the small integrin-binding ligand N-linked glycoprotein (SIBLING) family. Originally isolated from bone, OPN has been found in kidneys, vascular tissues, biological fluids, and various tumor tissues. OPN can interact with CD44 and integrins and regulate diverse biological processes. It has a multifaceted role in bone development and remodeling, and is also involved in the inflammatory and immune response, oncogenesis and cancer progression.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | SPP1,BNSP,Bone sialoprotein 1,BSPI,ETA 1 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
Publications
| Species | Sample Type | Title |
|---|---|---|
Cancer Commun (Lond) Hypoxia-Induced Osteopontin-Positive Glioma-Associated Macrophages Facilitate Glioma Mesenchymal Transition via NF-κB Pathway Activation. | ||
Cancer Med LILRB1 Is a Prognostic-Related Biomarker Correlated With Immune Infiltration in Head-Neck Squamous Cell Carcinoma. |