Human PARL ELISA Kit
Cat no : KE01007
Synonyms
EC:3.4.21.105, Pbeta, P-beta, Presenilin-associated rhomboid-like protein, mitochondrial, PRO2207
Validation Data Gallery
Product Information
KE01007 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human PARL ELISA kit is to be used to detect and quantify protein levels of endogenous human PARL. The assay recognizes human PARL. An antibody specific for human PARL has been pre-coated onto the microwells. The human PARL protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human PARL is added to detect the captured human PARL protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human PARL ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.01 ng/mL |
| Range | 0.78-50 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 55486 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 112% | 99%-122% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 25.13 | 1.51 | 6.01 |
| 2 | 8 | 5.86 | 0.29 | 4.95 |
| 3 | 8 | 3.29 | 0.24 | 7.44 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 25.64 | 1.46 | 5.69 |
| 2 | 16 | 5.84 | 0.27 | 4.67 |
| 3 | 16 | 3.16 | 0.29 | 9.20 |
Background Information
PARL is a member of the Rhomboid family of regulated intramembrane proteases (RIP), which are integral membrane enzymes that catalyze peptide bond hydrolysis of membrane-anchored substrates through cleavage of either the transmembrane spanning helix or adjacent region. PARL regulates mitochondrial remodeling and apoptosis through regulated substrate proteolysis.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | EC:3.4.21.105, Pbeta, P-beta, Presenilin-associated rhomboid-like protein, mitochondrial, PRO2207 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.