Human PBRM1 ELISA Kit
Cat no : KE00669
Synonyms
BAF180, BRG1-associated factor 180, hPB1, PB1, PBRM 1
Validation Data Gallery
Product Information
KE00669 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human PBRM1 ELISA kit is to be used to detect and quantify protein levels of endogenous human PBRM1. The assay recognizes human PBRM1. An antibody specific for human PBRM1 has been pre-coated onto the microwells. The human PBRM1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human PBRM1 is added to detect the captured human PBRM1 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human PBRM1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.07 ng/mL |
| Range | 0.313-20 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 55193 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 91% | 90%-93% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 8.83 | 0.82 | 9.32 |
| 2 | 8 | 2.26 | 0.12 | 5.41 |
| 3 | 8 | 1.14 | 0.06 | 4.89 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 8.41 | 0.91 | 10.86 |
| 2 | 16 | 2.17 | 0.17 | 7.92 |
| 3 | 16 | 1.10 | 0.08 | 7.55 |
Background Information
PBRM1 (or BAF180) is a subunit of the PBAF chromatin remodelling complex, one of three mammalian SWI/SNF remodelling complexes. PBRM1 is one of the most frequently altered genes in cancer. Loss of function mutations are particularly prevalent in clear cell renal cell carcinoma (ccRCC), but they are also found across a range of other cancer types.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | BAF180, BRG1-associated factor 180, hPB1, PB1, PBRM 1 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.