Human PDGFR alpha/CD140a ELISA Kit
Cat no : KE00758
Synonyms
PDGFRA, Alpha platelet-derived growth factor receptor, Alpha-type platelet-derived growth factor receptor, CD140 antigen-like family member A, CD140A
Validation Data Gallery
Product Information
KE00758 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human PDGFR alpha/CD140a ELISA kit is to be used to detect and quantify protein levels of endogenous human PDGFR alpha/CD140a. The assay recognizes human PDGFR alpha/CD140a. An antibody specific for human PDGFR alpha/CD140a has been pre-coated onto the microwells. The human PDGFR alpha/CD140a protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human PDGFR alpha/CD140a is added to detect the captured human PDGFR alpha/CD140a protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human PDGFR alpha/CD140a ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell lysate, Tissue lysate |
| Assay type | Sandwich |
| Sensitivity | 1.2 pg/mL |
| Range | 78.1-5000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 5156 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 98% | 94%-103% |
| Lysate | 95% | 92%-100% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 2,347.8 | 60.4 | 2.6 |
| 2 | 8 | 508.7 | 13.8 | 2.7 |
| 3 | 8 | 264.8 | 7.0 | 2.6 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 2,317.5 | 88.1 | 3.8 |
| 2 | 16 | 516.3 | 19.4 | 3.8 |
| 3 | 16 | 270.8 | 8.7 | 3.2 |
Background Information
PDGFR alpha (Platelet-derived growth factor receptor alpha), also known as CD140a, is a cell surface receptor tyrosine kinase that plays a crucial role in various biological processes. PDGFR alpha has been shown to regulate angiogenesis both in normal and pathological conditions, mainly stimulating the production of vascular endothelial growth factor (VEGF). Moreover, PDGFR alpha expression is correlated with aggressive disease and significantly worse patient survival in pediatric high-grade gliomas and hepatocellular carcinoma.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | PDGFRA, Alpha platelet-derived growth factor receptor, Alpha-type platelet-derived growth factor receptor, CD140 antigen-like family member A, CD140A |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.