Human PDGFR beta ELISA Kit
Cat no : KE00331
Synonyms
CD140B, JTK12, PDGF R beta, PDGFR, PDGFR1, PDGFRB
Validation Data Gallery
Product Information
KE00331 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human PDGFR beta ELISA kit is to be used to detect and quantify protein levels of endogenous human PDGFR beta. The assay recognizes human PDGFR beta. An antibody specific for human PDGFR beta has been pre-coated onto the microwells. The human PDGFR beta protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human PDGFR beta is added to detect the captured human PDGFR beta protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human PDGFR beta ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Tissue Lysate |
| Assay type | Sandwich |
| Sensitivity | 0.01 ng/mL |
| Range | 0.156-10 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 5159 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 111% | 96%-129% |
| Tissue lysate | 93% | 73%-125% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 3.74 | 0.14 | 3.86 |
| 2 | 20 | 0.92 | 0.04 | 4.42 |
| 3 | 20 | 0.22 | 0.02 | 8.93 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 3.64 | 0.17 | 4.69 |
| 2 | 24 | 0.89 | 0.03 | 3.56 |
| 3 | 24 | 0.23 | 0.01 | 5.56 |
Background Information
PDGFR beta(platelet-derived growth factor receptor beta) is a typical transmembrane tyrosine kinase receptor that promotes blood vessel formation when activated by platelet-derived growth factor (PDGF) and controls many important cellular processes such as growth, proliferation, movement, and survival. PDGFR beta is expressed in neurons, vascular smooth muscle cells, and pericytes. PDGFR beta may serve as a prognostic factor in GC (Gastric cancer) by immunohistochemical expression. In addition, PDGFR beta activation is sufficient to promote cardiomyocyte proliferation and heart regeneration by stimulating EZH2-mediated Ink4a/Arf repression.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CD140B, JTK12, PDGF R beta, PDGFR, PDGFR1, PDGFRB |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.