Human PGA5 ELISA Kit
Cat no : KE00084
Synonyms
Pepsin A, PGA3, PGA4, PGA5
Validation Data Gallery
Product Information
KE00084 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The PGA5 ELISA kit is to be used to detect and quantify protein levels of endogenous PGA5. The assay recognizes human PGA5. An antibody specific for PGA5 has been pre-coated onto the microwells. The PGA5 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for PGA5 is added to detect the captured PGA5 protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human PGA5 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 0.16 ng/mL |
| Range | 0.5-32 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 5222 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 93% | 75%-115% |
| Cell culture supernatants | 104% | 78%-127% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 24.36 | 1.33 | 5.5 |
| 2 | 20 | 6.43 | 0.33 | 5.2 |
| 3 | 20 | 0.65 | 0.06 | 8.9 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 23.79 | 1.86 | 7.8 |
| 2 | 24 | 5.78 | 0.49 | 8.5 |
| 3 | 24 | 0.48 | 0.04 | 9.0 |
Background Information
Pepsinogen I belongs to the peptidase A1 family. The gene endoces a 388 amino acid protein with a 15 amino acid signal peptide and 46 amino acid propeptide. Pepsinogen I is secreted only by the chief cells of the corpus, and its serum level decreases with increasing grade of the corpus atrophy (loss of oxyntic glands). Low serum levels of pepsinogen I are indicative of chronic atrophy, a risk factor for gastric cancer.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Pepsin A, PGA3, PGA4, PGA5 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.