Human PI4K2A ELISA Kit
Cat no : KE01016
Synonyms
EC:2.7.1.67, Phosphatidylinositol 4-kinase type 2-alpha, Phosphatidylinositol 4-kinase type II-alpha, PI 4-kinase II alpha, PI4KII
Validation Data Gallery
Product Information
KE01016 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human PI4K2A ELISA kit is to be used to detect and quantify protein levels of endogenous human PI4K2A. The assay recognizes human PI4K2A. An antibody specific for human PI4K2A has been pre-coated onto the microwells. The human PI4K2A protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human PI4K2A is added to detect the captured human PI4K2A protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human PI4K2A ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.15 ng/mL |
| Range | 0.78-50 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 55361 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 84% | 72%-109% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 24.32 | 0.80 | 3.30 |
| 2 | 8 | 6.17 | 0.15 | 2.40 |
| 3 | 8 | 3.08 | 0.06 | 1.95 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 23.45 | 1.34 | 5.73 |
| 2 | 16 | 5.77 | 0.48 | 8.28 |
| 3 | 16 | 3.00 | 0.12 | 3.96 |
Background Information
PI4K2A (phosphatidylinositol 4-kinase type 2 alpha) is a key lipid kinase whose main functions include catalyzing phosphatidylinositol phosphorylation, regulating lipid droplet formation and lipid storage, and participating in lysosomal damage repair. It plays multiple roles in cell signaling, lipid metabolism, membrane trafficking and organelle homeostasis.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | EC:2.7.1.67, Phosphatidylinositol 4-kinase type 2-alpha, Phosphatidylinositol 4-kinase type II-alpha, PI 4-kinase II alpha, PI4KII |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.