Human PISD ELISA Kit
Cat no : KE00759
Synonyms
EC:4.1.1.65, Phosphatidylserine decarboxylase alpha chain, Phosphatidylserine decarboxylase beta chain, Phosphatidylserine decarboxylase proenzyme, mitochondrial, PSDC
Validation Data Gallery
Product Information
KE00759 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human PISD ELISA kit is to be used to detect and quantify protein levels of endogenous human PISD. The assay recognizes human PISD. An antibody specific for human PISD has been pre-coated onto the microwells. The human PISD protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human PISD is added to detect the captured human PISD protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human PISD ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.12 ng/mL |
| Range | 0.78-50 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 23761 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 102% | 90%-115% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 22.60 | 0.86 | 3.80 |
| 2 | 8 | 5.99 | 0.27 | 4.51 |
| 3 | 8 | 2.63 | 0.11 | 4.24 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 22.63 | 1.46 | 6.46 |
| 2 | 16 | 5.49 | 0.53 | 9.60 |
| 3 | 16 | 2.41 | 0.25 | 10.26 |
Background Information
PISD (Phosphatidylserine decarboxylase proenzyme) belongs to the phosphatidylserine decarboxylase family. It contains a conserved LGST motif that functions as an autocatalytic cleavage site where the proenzyme is split into mature alpha and beta subunits. It has 2 isoforms produced by alternative splicing. PISD protein is mainly located in the inner membrane of mitochondria and catalyzes the conversion of phosphatidylserine (PS) to phosphatidylethanolamine (PE).
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | EC:4.1.1.65, Phosphatidylserine decarboxylase alpha chain, Phosphatidylserine decarboxylase beta chain, Phosphatidylserine decarboxylase proenzyme, mitochondrial, PSDC |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.