Human POMP ELISA Kit
Cat no : KE00536
Synonyms
POMP, C13orf12, HSPC014, HSPC036, hUMP1
Validation Data Gallery
Product Information
KE00536 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human POMP ELISA kit is to be used to detect and quantify protein levels of endogenous human POMP. The assay recognizes human POMP. An antibody specific for human POMP has been pre-coated onto the microwells. The human POMP protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human POMP is added to detect the captured human POMP protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human POMP ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.01 ng/mL |
| Range | 0.234-15 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 51371 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 75% | 70%-77% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 7.67 | 0.44 | 5.76 |
| 2 | 8 | 1.97 | 0.08 | 3.99 |
| 3 | 8 | 0.90 | 0.05 | 6.06 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 7.51 | 0.47 | 6.31 |
| 2 | 16 | 1.85 | 0.17 | 9.39 |
| 3 | 16 | 0.89 | 0.06 | 6.96 |
Background Information
Proteasome maturation protein(POMP), is an essential factor of mammalian proteasome biogenesis. It facilitates the main steps in 20S core complex formation at the ER to coordinate the assembly process and to provide cells with freshly formed proteasomes at their site of function. A single nucleotide deletion in this gene causes an autosomal recessive skin disorder, keratosis linearis with ichthyosis congenita and sclerosing keratoderma (KLICK) syndrome. POMP is strongly and consistently expressed from the basal to the granular layer of the epidermis in healthy people, whereas in KLICK patients, the staining is strong in the basal layer with a gradual decrease toward the granular layer.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | POMP, C13orf12, HSPC014, HSPC036, hUMP1 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.