Human PRAME ELISA Kit
Cat no : KE01000
Synonyms
MAPE, OIP 4, OIP4, OIP-4, Opa-interacting protein 4
Validation Data Gallery
Product Information
KE01000 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human PRAME ELISA kit is to be used to detect and quantify protein levels of endogenous human PRAME. The assay recognizes human PRAME. An antibody specific for human PRAME has been pre-coated onto the microwells. The human PRAME protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human PRAME is added to detect the captured human PRAME protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human PRAME ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.69 ng/mL |
| Range | 3.13-200 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 23532 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 72% | 71%-74% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 94.99 | 7.51 | 7.91 |
| 2 | 8 | 22.47 | 1.67 | 7.44 |
| 3 | 8 | 6.25 | 0.50 | 7.97 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 89.91 | 9.66 | 10.74 |
| 2 | 16 | 21.87 | 1.81 | 8.27 |
| 3 | 16 | 6.21 | 0.50 | 8.08 |
Background Information
PRAME (PReferentially expressed Antigen in MElanoma) is a tumor-associated antigen that was first identified through analysis of the specificity of tumor-reactive T-cell clones derived from a patient with metastatic cutaneous melanoma. It was subsequently found that PRAME is not only expressed in cutaneous melanoma, but also ocular melanoma and various nonmelanocytic malignant neoplasms, including non-small cell lung cancer, breast carcinoma, renal cell carcinoma, ovarian carcinoma, leukemia, synovial sarcoma, and myxoid liposarcoma.Normal healthy tissues are not known to express PRAME except for testis, ovary, placenta, adrenals, and endometrium. The expression of PRAME is a marker for an undifferentiated cellular state and has been implicated in the inhibition of tumour cell differentiation and the promotion of proliferation, which can contribute to oncogenesis .
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | MAPE, OIP 4, OIP4, OIP-4, Opa-interacting protein 4 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.