Human PROCR/EPCR/CD201 ELISA Kit
Cat no : KE00646
Synonyms
CD201, EPCR, PROCR, Activated protein C receptor, APC receptor
Validation Data Gallery
Product Information
KE00646 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human PROCR/EPCR/CD201 ELISA kit is to be used to detect and quantify protein levels of endogenous human PROCR/EPCR/CD201. The assay recognizes human PROCR/EPCR/CD201. An antibody specific for human PROCR/EPCR/CD201 has been pre-coated onto the microwells. The human PROCR/EPCR/CD201 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human PROCR/EPCR/CD201 is added to detect the captured human PROCR/EPCR/CD201 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human PROCR/EPCR/CD201 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma |
| Assay type | Sandwich |
| Sensitivity | 6.2 pg/mL |
| Range | 31.25-2000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 10544 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 108% | 102%-121% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 1,021.6 | 36.1 | 3.5 |
| 2 | 8 | 261.8 | 10.5 | 4.0 |
| 3 | 8 | 131.9 | 8.0 | 6.1 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 1,001.0 | 40.6 | 4.1 |
| 2 | 16 | 250.9 | 20.2 | 8.1 |
| 3 | 16 | 139.6 | 20.6 | 14.8 |
Background Information
EPCR (endothelial cell protein C receptor), also known as CD201, is a type 1 transmembrane protein that is primarily found on endothelial cells. It exhibits sequence and three-dimensional structural homology with the major histocompatibility class 1/CD1 family of proteins, particularly CD1d. It binds activated protein C and enhances protein C activation by the thrombin-thrombomodulin complex. It plays a role in the protein C pathway controlling blood coagulation.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CD201, EPCR, PROCR, Activated protein C receptor, APC receptor |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH franck (Verified Customer) (06-03-2026) | The ELISA assay is very quick and simple. Detection was performed in conditioned media from cultured human aortic endothelial cells as well as in cell lysates (RIPA lysis).
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