Human PROS1 ELISA Kit
Cat no : KE00899
Synonyms
PROS, Vitamin K-dependent protein S
Validation Data Gallery
Product Information
KE00899 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human PROS1 ELISA kit is to be used to detect and quantify protein levels of endogenous human PROS1. The assay recognizes human PROS1. An antibody specific for human PROS1 has been pre-coated onto the microwells. The human PROS1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human PROS1 is added to detect the captured human PROS1 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human PROS1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.08 ng/mL |
| Range | 0.625-40 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 5627 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 101% | 89%-119% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 22.97 | 0.72 | 3.15 |
| 2 | 8 | 5.11 | 0.21 | 4.14 |
| 3 | 8 | 2.82 | 0.25 | 8.99 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 23.18 | 1.26 | 5.44 |
| 2 | 16 | 4.94 | 0.27 | 5.46 |
| 3 | 16 | 2.87 | 0.24 | 8.29 |
Background Information
PROS1, also named as Vitamin K-dependent protein S, is a 676 amino acid protein, which contains 4 EGF-like domains, contains one Gla (gamma-carboxy-glutamate) domain and contains 2 laminin G-like domains. PROS1 as a secreted protein platelets alpha granules is expressed in plasma. PROS1 is a cofactor to activated protein C in the degradation of coagulation factors Va and VIIIa and helps to prevent coagulation and stimulating fibrinolysis.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | PROS, Vitamin K-dependent protein S |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.