Human PTBP2 ELISA Kit
Cat no : KE00688
Synonyms
brPTB, Neural polypyrimidine tract-binding protein, Neurally-enriched homolog of PTB, Polypyrimidine tract-binding protein 2, PTB
Validation Data Gallery
Product Information
KE00688 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human PTBP2 ELISA kit is to be used to detect and quantify protein levels of endogenous human PTBP2. The assay recognizes human PTBP2. An antibody specific for human PTBP2 has been pre-coated onto the microwells. The human PTBP2 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human PTBP2 is added to detect the captured human PTBP2 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human PTBP2 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.7 pg/mL |
| Range | 3.13-200 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 58155 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 100% | 87%-107% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 105.1 | 6.6 | 6.3 |
| 2 | 8 | 24.4 | 1.6 | 6.7 |
| 3 | 8 | 6.8 | 0.5 | 7.9 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 101.6 | 7.1 | 7.0 |
| 2 | 16 | 23.7 | 1.6 | 6.7 |
| 3 | 16 | 6.6 | 0.5 | 7.0 |
Background Information
PTBP2 (Polypyrimidine tract binding protein 2), also named as NPTB, PTB and PTBLP, is a RNA-binding protein which binds to intronic polypyrimidine tracts and mediates negative regulation of exons splicing. It may antagonize in a tissue-specific manner the ability of NOVA1 to activate exon selection. PTBP2 significantly increases in spermatocytes and persists until the elongating steps of spermatid differentiation. Beside, PTBP2 is required for neuron development and survival and functions in part to suppress adult splicing patterns to control the temporal regulation of neuronal maturation.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | brPTB, Neural polypyrimidine tract-binding protein, Neurally-enriched homolog of PTB, Polypyrimidine tract-binding protein 2, PTB |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.