Speedy™ Human Pentraxin 3/TSG-14 One-Step ELISA Kit
Cat no : SE50022
Synonyms
Pentraxin 3, PTX3, Pentaxin related protein PTX3, Pentaxin-related protein PTX3, Pentraxin related protein PTX3
Validation Data Gallery
Product Information
| Product name | Speedy™ Human Pentraxin 3/TSG-14 One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum,Plasma,Cell culture supernatants,Saliva,Cell lysates |
| Assay type | Sandwich |
| Sensitivity | 0.1 pg/mL |
| Range | 23.4-1500 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 5806 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell culture supernatant | 87% | 84%-91% |
| Saliva | 100% | 85%-129% |
| Cell lysate | 79% | 73%-90% |
| Human plasma | 95% | 78%-107% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 793.1 | 49.4 | 6.2 |
| 2 | 8 | 176.5 | 5.6 | 3.2 |
| 3 | 8 | 88.5 | 6.3 | 7.1 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 782.1 | 42.5 | 5.4 |
| 2 | 16 | 177.2 | 8.0 | 4.5 |
| 3 | 16 | 86.1 | 5.2 | 6.0 |
Background Information
Pentraxin 3 (PTX3), also known as TNF-inducible gene 14 protein (TSG-14), is a member of the pentraxin superfamily which consists of evolutionarily conserved proteins characterized by a structural motif, the pentraxin domain. Pentraxin 3 can be produced by a variety of cell types including endothelial cells, smooth muscle cells, adipocytes, fibroblasts, mononuclear phagocytes, and dendritic cells, and its expression is induced by primary inflammatory signals, such as IL-1, TNF, and microbial moieties. Pentraxin 3 is an acute phase glycoprotein that plays a role in the regulation of innate resistance to pathogens, inflammation, tissue remodeling and repair, female fertility and cancer. Pentraxin 3 is a biomarker for cardiovascular disease. Increased plasma Pentraxin 3 levels have been found in patients with cardiovascular disorders.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Pentraxin 3, PTX3, Pentaxin related protein PTX3, Pentaxin-related protein PTX3, Pentraxin related protein PTX3 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
2、If thermostatic shaker is not available, will it affect the duration of my experiment and the experimental data?
Answer: If thermostatic shaker is not available, it may take 2.5 hours to complete this experiment. After adding the antibody cocktail, we suggest you incubate the plate at 37℃ for 2 hours. After adding TMB, you only need to incubate the plate at 37℃ for 15 minutes. Through comparative experiments, our R&D personnel have confirmed that you can still obtain valid experimental data, although the signal will be slightly reduced.