Human RAD51C ELISA Kit
Cat no : KE00819
Synonyms
Rad 51C, RAD51 homolog C, RAD51 like protein 2
Validation Data Gallery
Product Information
KE00819 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human RAD51C ELISA kit is to be used to detect and quantify protein levels of endogenous human RAD51C. The assay recognizes human RAD51C. An antibody specific for human RAD51C has been pre-coated onto the microwells. The human RAD51C protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human RAD51C is added to detect the captured human RAD51C protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human RAD51C ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysates |
| Assay type | Sandwich |
| Sensitivity | 0.02 ng/mL |
| Range | 0.156-10 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 5889 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 104% | 101%-106% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 4.90 | 0.14 | 2.76 |
| 2 | 8 | 1.10 | 0.05 | 4.51 |
| 3 | 8 | 0.64 | 0.01 | 2.33 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 5.10 | 0.39 | 7.63 |
| 2 | 16 | 1.12 | 0.05 | 4.11 |
| 3 | 16 | 0.62 | 0.03 | 4.79 |
Background Information
RAD51C (DNA repair protein RAD51 homolog 3) is essential for the homologous recombination (HR) pathway of DNA repair and is involved in the homologous recombination repair (HRR) pathway of double-stranded DNA breaks arising during DNA replication or induced by DNA-damaging agents. It also contributes to DNA cross-link resistance, sister chromatid cohesion and genomic stability, and is involved in maintaining centrosome number in mitosis.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Rad 51C, RAD51 homolog C, RAD51 like protein 2 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.