Human RAE1 ELISA Kit
Cat no : KE00534
Synonyms
RAE1, MIG14, Mnrp41, mRNA export factor, mRNA export factor RAE1
Validation Data Gallery
Product Information
KE00534 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human RAE1 ELISA kit is to be used to detect and quantify protein levels of endogenous human RAE1. The assay recognizes human RAE1. An antibody specific for human RAE1 has been pre-coated onto the microwells. The human RAE1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human RAE1 is added to detect the captured human RAE1 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human RAE1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.02 ng/mL |
| Range | 0.156-10 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 8480 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 92% | 79%-101% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 5.18 | 0.25 | 4.91 |
| 2 | 8 | 1.38 | 0.04 | 3.10 |
| 3 | 8 | 0.66 | 0.03 | 4.41 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 5.07 | 0.30 | 6.01 |
| 2 | 16 | 1.32 | 0.09 | 6.53 |
| 3 | 16 | 0.66 | 0.02 | 3.67 |
Background Information
RAE1 is a protein involved in the transport of mRNA from the nucleus to the cytoplasm. It is highly conserved across species and plays a crucial role in various biological processes. RAE1 has been implicated in various diseases, particularly in cancer. For example, high expression of RAE1 in gastric cancer (GC) is associated with poor prognosis and promotes tumor cell proliferation, migration, and invasion through the ERK/MAPK signaling pathway.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | RAE1, MIG14, Mnrp41, mRNA export factor, mRNA export factor RAE1 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.