Human RAF1 ELISA Kit
Cat no : KE00842
Synonyms
c Raf, CRAF, EC:2.7.11.1, NS5, Proto oncogene c RAF
Validation Data Gallery
Product Information
KE00842 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human NCX1 ELISA kit is to be used to detect and quantify protein levels of endogenous human NCX1. The assay recognizes human NCX1. An antibody specific for human NCX1 has been pre-coated onto the microwells. The human NCX1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human NCX1 is added to detect the captured human NCX1 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human RAF1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 3.4 pg/mL |
| Range | 19.5-1250 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 5894 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 98% | 90%-103% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 604.1 | 29.1 | 4.8 |
| 2 | 8 | 177.4 | 1.7 | 1.0 |
| 3 | 8 | 56.9 | 1.4 | 2.5 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 609.7 | 29.5 | 4.8 |
| 2 | 16 | 169.8 | 8.6 | 5.1 |
| 3 | 16 | 53.8 | 3.9 | 7.3 |
Background Information
RAF1, also known as Raf-1 proto-oncogene serine/threonine kinase, plays pivotal roles in the MAPK/ERK signaling pathway. It is activated by growth factor receptors and other extracellular stimuli, promoting cell proliferation, differentiation, and survival. Upon activation, RAF1 phosphorylates and activates MEK, which in turn activates ERK. Abnormal activation of RAF1 is implicated in various cancers. Mutations or overexpression of RAF1 can lead to constitutive activation of the MAPK pathway, driving cell proliferation and tumorigenesis. For example, RAF1 is involved in the development of multiple tumors, such as non-small cell lung cancer.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | c Raf, CRAF, EC:2.7.11.1, NS5, Proto oncogene c RAF |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.