Human RAPGEF2 ELISA Kit
Cat no : KE00591
Synonyms
CNrasGEF, KIAA0313, nRap GEP, NRAPGEP, PDZ GEF1
Validation Data Gallery
Product Information
KE00591 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human RAPGEF2 ELISA kit is to be used to detect and quantify protein levels of endogenous human RAPGEF2. The assay recognizes human RAPGEF2. An antibody specific for human RAPGEF2 has been pre-coated onto the microwells. The human RAPGEF2 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human RAPGEF2 is added to detect the captured human RAPGEF2 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human RAPGEF2 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.05 ng/mL |
| Range | 0.156-10 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 9693 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 98% | 96%-104% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 5.88 | 0.23 | 3.83 |
| 2 | 8 | 1.61 | 0.07 | 4.43 |
| 3 | 8 | 0.41 | 0.03 | 7.15 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 6.02 | 0.30 | 5.02 |
| 2 | 16 | 1.59 | 0.07 | 4.66 |
| 3 | 16 | 0.40 | 0.03 | 7.96 |
Background Information
RAPGEF2 (also known as CNrasGEF, nRapGEP, PDZ-GEF1 and RA-GEF-1) is a neuronal-specific activator of Rap1 and Rap2 and associates with the synaptic scaffolding protein S-SCAM. It is a protein that plays a critical role in intracellular signalling pathways. RAPGEF2 is ubiquitously expressed in various tissues, including brain and bone marrow. RAPGEF2 has been implicated in several cancers and is implicated in synaptic loss and cognitive dysfunction in Alzheimer's disease models.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CNrasGEF, KIAA0313, nRap GEP, NRAPGEP, PDZ GEF1 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.