Human RBM20 ELISA Kit
Cat no : KE00581
Synonyms
RNA binding motif protein 20, RNA binding protein
Validation Data Gallery
Product Information
KE00581 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human RBM20 ELISA kit is to be used to detect and quantify protein levels of endogenous human RBM20. The assay recognizes human RBM20. An antibody specific for human RBM20 has been pre-coated onto the microwells. The human RBM20 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human RBM20 is added to detect the captured human RBM20 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human RBM20 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.1 pg/mL |
| Range | 78.1-5000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 282996 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 121% | 112%-129% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 1,836.3 | 154.7 | 8.4 |
| 2 | 8 | 674.2 | 22.8 | 3.4 |
| 3 | 8 | 360.0 | 28.8 | 8.0 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 1,889.6 | 149.0 | 7.9 |
| 2 | 16 | 702.1 | 46.1 | 6.6 |
| 3 | 16 | 657.8 | 45.1 | 6.9 |
Background Information
RBM20 (RNA binding motif protein 20) is a striated muscle-specific splicing factor that regulates alternative splicing of many cardiac genes encoding sarcomere and calcium-regulatory proteins. RBM20 has been identified as a crucial RNA-binding protein that controls the splicing of TTN. RBM20 is expressed in all striated muscles but is highest in cardiac tissue.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | RNA binding motif protein 20, RNA binding protein |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.