Human RIOK3 ELISA Kit
Cat no : KE00922
Synonyms
EC:2.7.11.1, RIO kinase 3, RIO kinase 3 (yeast), Serine/threonine-protein kinase RIO3, SUDD
Validation Data Gallery
Product Information
KE00922 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human RIOK3 ELISA kit is to be used to detect and quantify protein levels of endogenous human RIOK3. The assay recognizes human RIOK3. An antibody specific for human RIOK3 has been pre-coated onto the microwells. The human RIOK3 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human RIOK3 is added to detect the captured human RIOK3 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human RIOK3 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 15.3 pg/mL |
| Range | 125-8000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 8780 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 90% | 86%-93% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 3,965.8 | 146.3 | 3.7 |
| 2 | 8 | 1,088.6 | 35.8 | 3.3 |
| 3 | 8 | 537.5 | 23.1 | 4.3 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 4,165.0 | 268.5 | 6.4 |
| 2 | 16 | 1,046.3 | 54.4 | 5.2 |
| 3 | 16 | 533.7 | 26.6 | 5.0 |
Background Information
RIOK3, also known as RIO kinase 3, is an atypical serine/threonine protein kinase belonging to the RIO kinase family. It acts as an atypical kinase, meaning it doesn't follow the typical kinase structure and function patterns. It plays key roles in regulating innate immune responses, cell cycle progression, and tumorigenesis. RIOK3 modulates type I interferon production by phosphorylating downstream targets (e.g., TBK1, IRF3) and is implicated in inflammatory diseases and cancer, making it a potential therapeutic target for immune modulation and anticancer drug development.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | EC:2.7.11.1, RIO kinase 3, RIO kinase 3 (yeast), Serine/threonine-protein kinase RIO3, SUDD |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.