Human RNF40 ELISA Kit
Cat no : KE00636
Synonyms
95 kDa retinoblastoma-associated protein, BRE1 B, BRE1B, BRE1-B, E3 ubiquitin-protein ligase BRE1B
Validation Data Gallery
Product Information
KE00636 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human RNF40 ELISA kit is to be used to detect and quantify protein levels of endogenous human RNF40. The assay recognizes human RNF40. An antibody specific for human RNF40 has been pre-coated onto the microwells. The human RNF40 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human RNF40 is added to detect the captured human RNF40 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human RNF40 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 9.8 pg/mL |
| Range | 62.5-4000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 9810 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 114% | 109%-124% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 1,999.9 | 54.3 | 2.7 |
| 2 | 8 | 471.8 | 10.7 | 2.3 |
| 3 | 8 | 222.3 | 11.3 | 5.1 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 1,973.2 | 68.1 | 3.5 |
| 2 | 16 | 475.0 | 14.5 | 3.1 |
| 3 | 16 | 237.8 | 21.1 | 8.9 |
Background Information
RNF40 (Ring Finger Protein 40) is an E3 ubiquitin ligase, which is involved in the monoubiquitination of histone H2B at lysine 120 (H2Bub1), a modification that is crucial for transcriptional elongation and DNA repair. RNF20, RNF40 and H2Bub1 were also found to correlate with poor prognosis in hepatocellular carcinoma and to support the tumorigenic properties of prostate cancer and MLL-rearranged lymphoblastic leukemia.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | 95 kDa retinoblastoma-associated protein, BRE1 B, BRE1B, BRE1-B, E3 ubiquitin-protein ligase BRE1B |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.