Human RPP30 ELISA Kit
Cat no : KE00466
Synonyms
RPP30,RNase P subunit 2,RNaseP protein p30,RNASEP2
Validation Data Gallery
Product Information
KE00466 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human RPP30 ELISA kit is to be used to detect and quantify protein levels of endogenous human RPP30. The assay recognizes human RPP30. An antibody specific for human RPP30 has been pre-coated onto the microwells. The human RPP30 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human RPP30 is added to detect the captured human RPP30 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human RPP30 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.22 ng/mL |
| Range | 1.56-100 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 10556 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 95% | 85%-107% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 61.30 | 3.01 | 4.91 |
| 2 | 8 | 15.97 | 0.53 | 3.30 |
| 3 | 8 | 7.75 | 0.54 | 6.93 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 57.82 | 4.74 | 8.19 |
| 2 | 16 | 14.55 | 1.55 | 10.62 |
| 3 | 16 | 7.23 | 0.79 | 10.92 |
Background Information
RPP30 is part of the polyribonuclease P complex and the ribonuclease MRP complex, and is primarily involved in the 5' end maturation of tRNA, and may also be involved in other RNA metabolic processes, including RNA synthesis, processing, and maturation. RPP30 is widely expressed in a variety of tissues, including testis, lymph nodes, etc., and is also a potential biomarker for the diagnosis and prognosis of gastric cancer.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | RPP30,RNase P subunit 2,RNaseP protein p30,RNASEP2 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.