Human RPS17 ELISA Kit
Cat no : KE00473
Synonyms
RPS17,40S ribosomal protein S17,DBA4,ribosomal pro
Validation Data Gallery
Product Information
KE00473 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human RPS17 ELISA kit is to be used to detect and quantify protein levels of endogenous human RPS17. The assay recognizes human RPS17. An antibody specific for human RPS17 has been pre-coated onto the microwells. The human RPS17 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human RPS17 is added to detect the captured human RPS17 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human RPS17 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.01 ng/mL |
| Range | 0.156-10 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 6218 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 82% | 73%-89% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 4.69 | 0.32 | 6.86 |
| 2 | 8 | 1.23 | 0.02 | 1.33 |
| 3 | 8 | 0.55 | 0.04 | 6.71 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 4.89 | 0.31 | 6.31 |
| 2 | 16 | 1.16 | 0.09 | 7.73 |
| 3 | 16 | 0.58 | 0.04 | 7.66 |
Background Information
RPS17 (Ribosomal Protein S17) is a crucial component of the 40S ribosomal subunit, playing a vital role in protein synthesis. As a ribosomal protein, RPS17 is involved in the assembly and function of ribosomes, the cellular machinery responsible for translating mRNA into proteins. Furthermore, RPS17 has been implicated in several cancers, where its expression may be dysregulated, contributing to abnormal cell growth and proliferation.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | RPS17,40S ribosomal protein S17,DBA4,ribosomal pro |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.