Human RPS3 ELISA Kit
Cat no : KE00725
Synonyms
40S ribosomal protein S3, EC:4.2.99.18, FLJ26283, FLJ27450, OK/SW-cl.26
Validation Data Gallery
Product Information
KE00725 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human RPS3 ELISA kit is to be used to detect and quantify protein levels of endogenous human RPS3. The assay recognizes human RPS3. An antibody specific for human RPS3 has been pre-coated onto the microwells. The human RPS3 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human RPS3 is added to detect the captured human RPS3 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human RPS3 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.05 ng/mL |
| Range | 0.39-25 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 6188 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 117% | 112%-120% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 11.47 | 1.02 | 8.93 |
| 2 | 8 | 2.81 | 0.21 | 7.42 |
| 3 | 8 | 1.13 | 0.11 | 9.41 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 11.45 | 1.09 | 9.53 |
| 2 | 16 | 2.76 | 0.25 | 8.97 |
| 3 | 16 | 1.18 | 0.12 | 9.80 |
Background Information
RPS3 (Ribosomal Protein S3) is a multifunctional protein that plays a crucial role in protein synthesis as a component of the 40S ribosomal subunit. It is involved in various extraribosomal activities, including DNA repair, apoptosis, and the innate immune response to bacterial infection.RPS3 acts as a DNA repair endonuclease, participating in the repair of damaged DNA. It also plays a role in apoptosis, with its localization changing to the nuclear membrane during RPS3-induced apoptosis in lymphocytic cells. Additionally, RPS3 is involved in transcriptional regulation and has been implicated in processes such as cell proliferation and differentiation.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | 40S ribosomal protein S3, EC:4.2.99.18, FLJ26283, FLJ27450, OK/SW-cl.26 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.