Human/Rat NCX1 ELISA Kit
Cat no : KE00837
Synonyms
Na(+)/Ca(2+)-exchange protein 1, SLC8A1, Solute carrier family 8 member 1
Validation Data Gallery
Product Information
KE00837 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human/rat NCX1 ELISA kit is to be used to detect and quantify protein levels of endogenous human/rat NCX1. The assay recognizes human/rat NCX1. An antibody specific for human/rat NCX1 has been pre-coated onto the microwells. The human/rat NCX1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human/rat NCX1 is added to detect the captured human/rat NCX1 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human/Rat NCX1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Tissue lysate |
| Assay type | Sandwich |
| Sensitivity | 0.07 ng/mL |
| Range | 0.47-30 ng/mL |
| Reactivity | Human/Rat |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 6546 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Tissue lysate | 102% | 100%-107% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 16.41 | 0.37 | 2.27 |
| 2 | 8 | 3.98 | 0.29 | 7.24 |
| 3 | 8 | 1.91 | 0.14 | 7.18 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 15.78 | 0.92 | 5.80 |
| 2 | 16 | 3.78 | 0.32 | 8.43 |
| 3 | 16 | 1.90 | 0.15 | 7.82 |
Background Information
SLC8A1, also known as NCX1, belongs to the SLC (Solute Carrier) family and the CaCA (Calcium/Cation Antiporter) superfamily. NCX1 is a bidirectional ion exchanger that can exchange sodium and calcium ions between the inside and outside of cells in a 1:3 ratio (1 Ca2+ ion and 3 Na+ ions). Under physiological conditions, NCX1 mainly transports Ca2+ from the cell interior to the exterior through the forward transport mode to maintain the low calcium level inside the cell. However, under certain special conditions, such as ischemia-reperfusion injury and stroke, the reverse transport mode of NCX1 is activated, leading to the influx of Ca2+ into the cell, which may cause cell damage.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Na(+)/Ca(2+)-exchange protein 1, SLC8A1, Solute carrier family 8 member 1 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.