Human/Rat SURF1 ELISA Kit
Cat no : KE00673
Synonyms
SURF 1, SURF-1, surfeit 1, Surfeit locus protein 1
Validation Data Gallery
Product Information
KE00673 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human SURF1 ELISA kit is to be used to detect and quantify protein levels of endogenous human SURF1. The assay recognizes human SURF1. An antibody specific for human SURF1 has been pre-coated onto the microwells. The human SURF1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human SURF1 is added to detect the captured human SURF1 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human/Rat SURF1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate, Tissue lysate |
| Assay type | Sandwich |
| Sensitivity | 34.4 pg/mL |
| Range | 125-8000 pg/mL |
| Reactivity | Human/Rat |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 6834 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Lysate | 110% | 99%-126% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 1,698.5 | 76.6 | 4.5 |
| 2 | 8 | 673.8 | 52.4 | 7.8 |
| 3 | 8 | 396.7 | 21.5 | 5.4 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 1,556.6 | 158.3 | 10.2 |
| 2 | 16 | 681.8 | 93.9 | 13.8 |
| 3 | 16 | 400.6 | 19.3 | 4.8 |
Background Information
Surfeit locus protein 1 (SURF1) is a crucial component of the mitochondrial translation regulation assembly intermediate of cytochrome c oxidase complex (MITRAC complex). It plays a significant role in the biogenesis of cytochrome c oxidase (COX), a key enzyme in the mitochondrial electron transport chain responsible for oxidative phosphorylation. SURF1 aids in the correct assembly of COX by acting as a central assembly intermediate, receiving subunits imported to the inner mitochondrial membrane and regulating COX1 mRNA translation.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | SURF 1, SURF-1, surfeit 1, Surfeit locus protein 1 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.