Human Ribophorin I ELISA Kit
Cat no : KE00459
Synonyms
RPN1,Dolichyl-diphosphooligosaccharide--protein gl
Validation Data Gallery
Product Information
KE00459 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human Ribophorin I ELISA kit is to be used to detect and quantify protein levels of endogenous human Ribophorin I. The assay recognizes human Ribophorin I. An antibody specific for human Ribophorin I has been pre-coated onto the microwells. The human Ribophorin I protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human Ribophorin I is added to detect the captured human Ribophorin I protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human Ribophorin I ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 6.2 pg/mL |
| Range | 78.1-5000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 6184 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 77% | 72%-82% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 2,677.1 | 95.1 | 3.6 |
| 2 | 8 | 592.0 | 14.3 | 2.4 |
| 3 | 8 | 307.4 | 7.1 | 2.3 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 1,971.4 | 190.6 | 9.7 |
| 2 | 16 | 451.9 | 27.2 | 6.0 |
| 3 | 16 | 237.3 | 13.5 | 5.7 |
Background Information
Ribophorin I, also named as RPN1, is a key protein component of the oligosaccharyltransferase (OST) complex, which is embedded in the membrane of the rough endoplasmic reticulum (RER) (PMID: 38373565). It plays a crucial role in the process of N-linked glycosylation, a post-translational modification essential for the proper folding, stability, and function of many secretory and membrane-bound proteins (PMID: 17264154). Ribophorin I, along with Ribophorin II, forms a heterodimeric complex that facilitates the transfer of oligosaccharides from a lipid-linked donor to specific asparagine residues on nascent polypeptide chains (PMID: 30940778).
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | RPN1,Dolichyl-diphosphooligosaccharide--protein gl |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.