Human S1PR2 ELISA Kit
Cat no : KE00621
Synonyms
AGR16, EDG 5, EDG5, Endothelial differentiation G-protein coupled receptor 5, Gpcr13
Validation Data Gallery
Product Information
KE00621 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human S1PR2 ELISA kit is to be used to detect and quantify protein levels of endogenous human S1PR2. The assay recognizes human S1PR2. An antibody specific for human S1PR2 has been pre-coated onto the microwells. The human S1PR2 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human S1PR2 is added to detect the captured human S1PR2 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human S1PR2 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.08 ng/mL |
| Range | 1.56-100 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 9294 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 91% | 86%-96% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 45.41 | 2.12 | 4.67 |
| 2 | 8 | 11.02 | 0.39 | 3.57 |
| 3 | 8 | 6.04 | 0.14 | 2.23 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 45.48 | 1.73 | 3.81 |
| 2 | 16 | 11.04 | 0.30 | 2.76 |
| 3 | 16 | 6.22 | 0.40 | 6.45 |
Background Information
S1PR2 (sphingosine-1-phosphate receptor 2), also known as S1P2 or EDG5, is one of five G-protein-coupled receptors for sphingosine-1-phosphate (S1P), a biologically active metabolic product of sphingolipid. S1P and its receptors have important regulatory functions in normal physiology and disease processes, particularly involving the immune, central nervous, and cardiovascular systems. S1PR2 plays a role in acute vascular inflammation. S1PR2 signaling is implicated in STAT3 activation.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | AGR16, EDG 5, EDG5, Endothelial differentiation G-protein coupled receptor 5, Gpcr13 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.