Human SAE1 ELISA Kit
Cat no : KE00696
Synonyms
AOS1, HSPC140, Sentrin/SUMO activating protein AOS1, SUA1, SUMO-activating enzyme subunit 1
Validation Data Gallery
Product Information
KE00696 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human SAE1 ELISA kit is to be used to detect and quantify protein levels of endogenous human SAE1. The assay recognizes human SAE1. An antibody specific for human SAE1 has been pre-coated onto the microwells. The human SAE1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human SAE1 is added to detect the captured human SAE1 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human SAE1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.02 ng/mL |
| Range | 0.188-12 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 10055 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 99% | 96%-103% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 4.97 | 0.18 | 3.65 |
| 2 | 8 | 1.39 | 0.04 | 2.79 |
| 3 | 8 | 0.74 | 0.04 | 4.76 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 5.36 | 0.44 | 8.23 |
| 2 | 16 | 1.42 | 0.05 | 3.52 |
| 3 | 16 | 0.79 | 0.07 | 9.43 |
Background Information
SAE1(SUMO-activating enzyme subunit 1), also named as AOS1, SUA1, UBLE1A, is a component of a heterodimeric small ubiquitin-related modifier that plays a vital role in SUMOylation. SAE1 and UBA2 form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins. SAE1 is highly expressed in various malignant tumors and is closely associated with the tumorigenesis and tumor development. It is overexpressed in hepatocellular carcinoma and is associated with cancer metastasis, disease progression, and poor prognosis
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | AOS1, HSPC140, Sentrin/SUMO activating protein AOS1, SUA1, SUMO-activating enzyme subunit 1 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.