Speedy™ Human SALL4 One-Step ELISA Kit
Cat no : SE50101
Synonyms
DRRS, HSAL4, Sal like protein 4, Sal-like protein 4, Zinc finger protein 797
Validation Data Gallery
Product Information
| Product name | Speedy™ Human SALL4 One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma |
| Assay type | Sandwich |
| Sensitivity | 15.5 pg/mL |
| Range | 39-2500 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 57167 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 78% | 72%-84% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 1,303.6 | 26.6 | 2.0 |
| 2 | 8 | 345.3 | 6.5 | 1.9 |
| 3 | 8 | 95.4 | 4.8 | 5.0 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 1,304.1 | 23.1 | 1.8 |
| 2 | 16 | 344.5 | 6.6 | 1.9 |
| 3 | 16 | 95.4 | 5.1 | 5.3 |
Background Information
Spalt-Like Transcription Factor 4 (SALL4) is a zinc-finger transcriptional factor for embryonic stem cell self-renewal and pluripotency. SALL4 overexpression is found in several cancer tissues and hematologic malignancies,suggesting a key role of SALL4 in cancer onset and progression. Reactivation of SALL4 in cancerous cells is a key event in tumorigenesis and confers malignancy features to cells. The SALL4 protein contains several zinc finger cluster (ZFC) domains spread in different parts of the protein which give it both transcription activation and repression functions. SALL4 has two isoforms. SALL4A originates from full length transcript, while SALL4B as a spliced isoform lacks a part of exon 2. SALL4 abundancy is regulated by transcriptional and post-transcriptional mechanisms. Both SALL4A and SALL4B isoforms can bind β-catenin protein and synergistically activate the Wnt/β-catenin signaling pathway.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | DRRS, HSAL4, Sal like protein 4, Sal-like protein 4, Zinc finger protein 797 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
2、If thermostatic shaker is not available, will it affect the duration of my experiment and the experimental data?
Answer: If thermostatic shaker is not available, it may take 2.5 hours to complete this experiment. After adding the antibody cocktail, we suggest you incubate the plate at 37℃ for 2 hours. After adding TMB, you only need to incubate the plate at 37℃ for 15 minutes. Through comparative experiments, our R&D personnel have confirmed that you can still obtain valid experimental data, although the signal will be slightly reduced.