Human SCN3B ELISA Kit
Cat no : KE00675
Synonyms
HSA243396, KIAA1158, SCNB3, Sodium channel regulatory subunit beta-3, Sodium channel subunit beta 3
Validation Data Gallery
Product Information
KE00675 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human SCN3B ELISA kit is to be used to detect and quantify protein levels of endogenous human SCN3B. The assay recognizes human SCN3B. An antibody specific for human SCN3B has been pre-coated onto the microwells. The human SCN3B protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human SCN3B is added to detect the captured human SCN3B protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human SCN3B ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 4.6 pg/mL |
| Range | 78.1-5000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 55800 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 88% | 81%-91% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 2,350.7 | 36.4 | 1.5 |
| 2 | 8 | 542.2 | 12.0 | 2.2 |
| 3 | 8 | 291.2 | 21.8 | 7.5 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 2,302.3 | 66.8 | 2.9 |
| 2 | 16 | 541.4 | 12.4 | 2.3 |
| 3 | 16 | 286.8 | 16.0 | 5.6 |
Background Information
Sodium channel regulatory subunit beta-3 (SCN3B) is a regulatory subunit of the voltage-gated sodium channel complex. It modulates the inactivation kinetics of the sodium channel, which is crucial for the generation and propagation of action potentials in neurons and muscle cells. SCN3B has been shown to play a role in various cellular processes, including cell migration and apoptosis. In breast cancer, SCN3B has been identified as an anti-cancer molecule with migration inhibition effects. Additionally, SCN3B emerges as a promising potential prognostic biomarker for glioma, especially for oligodendroglioma.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | HSA243396, KIAA1158, SCNB3, Sodium channel regulatory subunit beta-3, Sodium channel subunit beta 3 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.