Human SHBG ELISA Kit
Cat no : KE00264
Synonyms
ABP, SBP, sex hormone binding globulin, Sex steroid binding protein, SHBG, TEBG
Validation Data Gallery
Product Information
KE00264 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The SHBG ELISA kit is to be used to detect and quantify protein levels of endogenous SHBG. The assay recognizes human SHBG. An antibody specific for SHBG has been pre-coated onto the microwells. The SHBG protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for SHBG is added to detect the captured SHBG protein. For signal development, Sterptravidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human SHBG ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum |
| Assay type | Sandwich |
| Sensitivity | 3.2 pmol/L |
| Range | 15.6-1000 pmol/L |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 6462 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Serum | 107% | 91%-123% |
IntraAssay
| Sample | n | mean ( pmol/L) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 32.0 | 2.0 | 6.3 |
| 2 | 20 | 116.8 | 4.3 | 3.7 |
| 3 | 20 | 439.6 | 31.1 | 7.1 |
InterAssay
| Sample | n | mean ( pmol/L) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 28.3 | 2.8 | 9.9 |
| 2 | 24 | 116.9 | 11.2 | 9.6 |
| 3 | 24 | 512.1 | 49.1 | 9.6 |
Background Information
SHBG (sex hormone-binding globulin), also known as androgen-binding protein (ABP), is the specific transport protein for sex steroid hormones in humans. SHBG is synthesized mainly by the liver and circulates in the blood. Alteration of SHBG levels have been linked to risks of insulin resistance, cardiovascular disease, male infertility and other diseases.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | ABP, SBP, sex hormone binding globulin, Sex steroid binding protein, SHBG, TEBG |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.