Human SLC6A6/TAUT ELISA Kit
Cat no : KE00562
Synonyms
SLC6A6, HTRDC, Sodium- and chloride-dependent taurine transporter, TAUT
Validation Data Gallery
Product Information
KE00562 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human SLC6A6/TAUT ELISA kit is to be used to detect and quantify protein levels of endogenous human SLC6A6/TAUT. The assay recognizes human SLC6A6/TAUT. An antibody specific for human SLC6A6/TAUT has been pre-coated onto the microwells. The human SLC6A6/TAUT protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human SLC6A6/TAUT is added to detect the captured human SLC6A6/TAUT protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human SLC6A6/TAUT ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.12 ng/mL |
| Range | 0.39-25 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 6533 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 92% | 85%-96% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 13.08 | 1.25 | 9.54 |
| 2 | 8 | 3.03 | 0.12 | 3.95 |
| 3 | 8 | 1.51 | 0.07 | 4.57 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 14.54 | 2.10 | 14.46 |
| 2 | 16 | 3.12 | 0.16 | 5.08 |
| 3 | 16 | 1.57 | 0.09 | 5.94 |
Background Information
SLC6A6, also known as the taurine transporter (TAUT), is a multi-pass membrane protein involved in the transport of taurine and beta-alanine. It plays a crucial role in maintaining cellular osmotic balance and has been implicated in various diseases. Elevated expression of SLC6A6 has been linked to tumorigenesis and poor clinical outcomes in gastric cancer. Additionally, mutations in SLC6A6 are associated with early retinal degeneration. Its function in transporting taurine also highlights its importance in immune responses and metabolic processes.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | SLC6A6, HTRDC, Sodium- and chloride-dependent taurine transporter, TAUT |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.