Human SMOC2 ELISA Kit
Cat no : KE00377
Synonyms
SMOC2,MSTP117,Secreted modular calcium-binding pro
Validation Data Gallery
Product Information
KE00377 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human SMOC2 ELISA kit is to be used to detect and quantify protein levels of endogenous human SMOC2. The assay recognizes human SMOC2. An antibody specific for human SMOC2 has been pre-coated onto the microwells. The human SMOC2 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human SMOC2 is added to detect the captured human SMOC2 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human SMOC2 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma |
| Assay type | Sandwich |
| Sensitivity | 15.0 pg/mL |
| Range | 62.5-4000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 64094 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Serum | 108% | 94%-116% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 1,932.0 | 87.7 | 4.5 |
| 2 | 20 | 476.3 | 20.5 | 4.3 |
| 3 | 20 | 121.4 | 6.8 | 5.6 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 1,774.9 | 113.1 | 6.4 |
| 2 | 24 | 450.5 | 37.7 | 8.4 |
| 3 | 24 | 148.2 | 13.8 | 9.3 |
Background Information
Secreted modular calcium-binding protein 2 (SMOC2), which is a member of the secreted protein acidic and rich in cysteine (SPARC) family of matricellular proteins, interacts with matrix proteins, cell surface receptors, cytokines, proteasomes and other effector molecules to regulate cell-cell and cell-microenvironment communication. It is expected to be located in extracellular space. The calculated molecular weight of SMOC2 is 50 kDa. In recent years, SMOC2 has been reported to be highly expressed in tumor tissues and to promote tumor migration, invasion and growth. In addition to its role in tumor progression, it also regulates wound healing, bone growth, fibrosis and embryogenesis.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | SMOC2,MSTP117,Secreted modular calcium-binding pro |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.