Human SMUG1 ELISA Kit
Cat no : KE00557
Synonyms
SMUG1, EC:3.2.2.-, FDG, HMUDG, Single-strand selective monofunctional uracil DNA glycosylase
Validation Data Gallery
Product Information
KE00557 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human SMUG1 ELISA kit is to be used to detect and quantify protein levels of endogenous human SMUG1. The assay recognizes human SMUG1. An antibody specific for human SMUG1 has been pre-coated onto the microwells. The human SMUG1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human SMUG1 is added to detect the captured human SMUG1 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human SMUG1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.10 ng/mL |
| Range | 0.313-20 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 23583 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 99% | 89%-109% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 12.02 | 0.62 | 5.20 |
| 2 | 8 | 2.87 | 0.18 | 6.20 |
| 3 | 8 | 0.70 | 0.05 | 7.00 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 11.57 | 0.88 | 7.59 |
| 2 | 16 | 2.88 | 0.13 | 4.51 |
| 3 | 16 | 0.74 | 0.05 | 7.44 |
Background Information
SMUG1 is a member of the uracil-DNA glycosylase (UDG) superfamily, which is involved in the base excision repair (BER) pathway. It specifically recognizes and removes uracil residues from single-stranded DNA, thereby preventing the accumulation of uracil in the genome. Uracil can be incorporated into DNA due to deamination of cytosine or during DNA replication errors. If left unrepaired, uracil can lead to mutations and genomic instability.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | SMUG1, EC:3.2.2.-, FDG, HMUDG, Single-strand selective monofunctional uracil DNA glycosylase |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.