Human SOD2 ELISA Kit
Cat no : KE00872
Synonyms
SODM, EC:1.15.1.1, IPO B, IPOB, Mn SOD
Validation Data Gallery
Product Information
KE00872 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human SOD2 ELISA kit is to be used to detect and quantify protein levels of endogenous human SOD2. The assay recognizes human SOD2. An antibody specific for human SOD2 has been pre-coated onto the microwells. The human SOD2 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human SOD2 is added to detect the captured human SOD2 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human SOD2 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.14 ng/mL |
| Range | 0.78-50 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 6648 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 117% | 100%-131% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 24.15 | 0.39 | 1.63 |
| 2 | 8 | 5.87 | 0.20 | 3.39 |
| 3 | 8 | 3.03 | 0.13 | 4.17 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 24.39 | 0.41 | 1.68 |
| 2 | 16 | 5.88 | 0.22 | 3.69 |
| 3 | 16 | 3.10 | 0.15 | 4.85 |
Background Information
SOD2 encodes the manganese superoxide dismutase (Mn-SOD), which is involved in regulating oxidative stress in the cell through the dismutation of superoxide radicals (O2−) to H2O2 and molecular oxygen . Reactive oxygen species (ROS) cause DNA damage, resulting in genetic aberrations and genomic instability, thus promoting mutagenesis and carcinogenesis. Alteration in the expression of antioxidant enzymes, like catalase (CAT), glutathione peroxidases (GPx), and superoxide dismutases (SOD), can impair regulation of enzymatic activity and modify ROS detoxification .
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | SODM, EC:1.15.1.1, IPO B, IPOB, Mn SOD |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.