Human SOX4 ELISA Kit
Cat no : KE00509
Synonyms
CSS10, EVI16, Transcription factor SOX-4
Validation Data Gallery
Product Information
KE00509 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human SOX4 ELISA kit is to be used to detect and quantify protein levels of endogenous human SOX4. The assay recognizes human SOX4. An antibody specific for human SOX4 has been pre-coated onto the microwells. The human SOX4 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human SOX4 is added to detect the captured human SOX4 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human SOX4 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 5.7 pg/mL |
| Range | 62.5-4000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 6659 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysates | 109% | 109%-110% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 1795.1 | 71.7 | 4.0 |
| 2 | 8 | 387.3 | 19.4 | 5.0 |
| 3 | 8 | 100.1 | 4.8 | 4.8 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 1,791.7 | 72.6 | 4.0 |
| 2 | 16 | 383.5 | 17.6 | 4.6 |
| 3 | 16 | 100.5 | 6.2 | 6.2 |
Background Information
SOX4 is a transcription factor belonging to the SOX family, which is crucial for embryonic development and cell fate determination. It is located on chromosome 6p22.3 in humans and encodes a protein of 474 amino acids with three distinguishable domains: a high mobility group (HMG) box, a glycine-rich region, and a serine-rich region. This protein regulates gene expression by binding to DNA and interacting with other proteins, thereby influencing cell proliferation, differentiation, and apoptosis. SOX4 has been implicated in various diseases, including cancer and atherosclerosis, due to its role in promoting cell proliferation and survival. For instance, SOX4 is highly expressed in several cancers, such as non-small cell lung cancer (NSCLC), and is associated with poor prognosis. It can induce epithelial-mesenchymal transition (EMT), enhancing cancer cell invasion and metastasis. Additionally, SOX4 contributes to atherosclerosis by regulating endothelial cell function. Its diverse functions and involvement in multiple signaling pathways make SOX4 a significant focus in both basic research and clinical studies.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CSS10, EVI16, Transcription factor SOX-4 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.